Functional identification of islet cell types by electrophysiological fingerprinting

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Functional identification of islet cell types by electrophysiological fingerprinting

The α-, β- and δ-cells of the pancreatic islet exhibit different electrophysiological features. We used a large dataset of whole-cell patch-clamp recordings from cells in intact mouse islets (N = 288 recordings) to investigate whether it is possible to reliably identify cell type (α, β or δ) based on their electrophysiological characteristics. We quantified 15 electrophysiological variables in ...

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Dendritic cells (DC) play important roles in both tolerance and immunity to β cells in type 1 diabetes. How and why DC can have diverse and opposing functions in islets remains elusive. To answer these questions, islet DC subsets and their specialized functions were characterized. Under both homeostatic and inflammatory conditions, there were two main tissue-resident DC subsets in islets, defin...

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PAX6 maintains β cell identity by repressing genes of alternative islet cell types.

Type 2 diabetes is thought to involve a compromised β cell differentiation state, but the mechanisms underlying this dysfunction remain unclear. Here, we report a key role for the TF PAX6 in the maintenance of adult β cell identity and function. PAX6 was downregulated in β cells of diabetic db/db mice and in WT mice treated with an insulin receptor antagonist, revealing metabolic control of exp...

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Comparative immunocytochemistry of isolated rat & monkey pancreatic islet cell types.

BACKGROUND & OBJECTIVES Data on comparative distribution of the islet cell types in the Indian bonnet monkeys and rats are not available. The aim of the present study was to compare the arrangement of the three islet cell types in the native and in isolated rat and Indian bonnet monkey islets by immunocytochemistry. METHODS Rat islets isolated by chopped tissue collagenase digestion method an...

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ژورنال

عنوان ژورنال: Journal of The Royal Society Interface

سال: 2017

ISSN: 1742-5689,1742-5662

DOI: 10.1098/rsif.2016.0999